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aav6 sirion biotech  (SIRION Biotech)

 
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    SIRION Biotech aav6 sirion biotech
    A Potential editing strategy for the generation of allogenic T cells. Multiplexed knock-out of TRAC , B2M , and CIITA to reduce the risk of host-versus-graft rejection and graft-versus-host disease responses, respectively. B Simultaneous triple knockout of TRAC , B2M , and CIITA (Class II Major Histocompatibility Complex Transactivator) by AsCas12a Ultra (1 µM RNP). The optimal guide RNA ( n = 1) targeting each locus was used for multiplex editing. Data are presented as mean values ± SD. C Workflow for generation of allogenic T cells with site-specific transgene knock-in by AsCas12a Ultra. The activated T cells were electroporated with Ultra RNPs and transduced with <t>AAV6</t> carrying a donor template post-electroporation, then analyzed 3–4 days later for transgene expression by flow cytometry. D The knock-in efficiency of a fluorescent reporter at either TRAC or B2M locus using 1 µM RNP and 1 × 10 5 vg/cell AAV6. The optimal gRNA targeting each locus (n = 1) was used for this experiment. Alternative RNPs (Alt RNP, i.e., TRAC RNP + B2M AAV or B2M RNP + TRAC AAV) were used as negative control to rule out any non-specific integration of AAV donor. Data are presented as mean values ± SD. Paired, two-tailed t-test was performed to evaluate statistical significance (** P -value = 0.0012; *** P -value = 0.0008). E Simultaneous double knock-in of fluorescent reporters at TRAC and B2M across a range of RNP concentrations. The optimal gRNA targeting each locus ( n = 1) was used for this experiment. Raw source data are provided in the Source Data File.
    Aav6 Sirion Biotech, supplied by SIRION Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav6+sirion+biotech/pmc08222333-361-11-12?v=SIRION+Biotech
    Average 90 stars, based on 1 article reviews
    aav6 sirion biotech - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines"

    Article Title: AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

    Journal: Nature Communications

    doi: 10.1038/s41467-021-24017-8

    A Potential editing strategy for the generation of allogenic T cells. Multiplexed knock-out of TRAC , B2M , and CIITA to reduce the risk of host-versus-graft rejection and graft-versus-host disease responses, respectively. B Simultaneous triple knockout of TRAC , B2M , and CIITA (Class II Major Histocompatibility Complex Transactivator) by AsCas12a Ultra (1 µM RNP). The optimal guide RNA ( n = 1) targeting each locus was used for multiplex editing. Data are presented as mean values ± SD. C Workflow for generation of allogenic T cells with site-specific transgene knock-in by AsCas12a Ultra. The activated T cells were electroporated with Ultra RNPs and transduced with AAV6 carrying a donor template post-electroporation, then analyzed 3–4 days later for transgene expression by flow cytometry. D The knock-in efficiency of a fluorescent reporter at either TRAC or B2M locus using 1 µM RNP and 1 × 10 5 vg/cell AAV6. The optimal gRNA targeting each locus (n = 1) was used for this experiment. Alternative RNPs (Alt RNP, i.e., TRAC RNP + B2M AAV or B2M RNP + TRAC AAV) were used as negative control to rule out any non-specific integration of AAV donor. Data are presented as mean values ± SD. Paired, two-tailed t-test was performed to evaluate statistical significance (** P -value = 0.0012; *** P -value = 0.0008). E Simultaneous double knock-in of fluorescent reporters at TRAC and B2M across a range of RNP concentrations. The optimal gRNA targeting each locus ( n = 1) was used for this experiment. Raw source data are provided in the Source Data File.
    Figure Legend Snippet: A Potential editing strategy for the generation of allogenic T cells. Multiplexed knock-out of TRAC , B2M , and CIITA to reduce the risk of host-versus-graft rejection and graft-versus-host disease responses, respectively. B Simultaneous triple knockout of TRAC , B2M , and CIITA (Class II Major Histocompatibility Complex Transactivator) by AsCas12a Ultra (1 µM RNP). The optimal guide RNA ( n = 1) targeting each locus was used for multiplex editing. Data are presented as mean values ± SD. C Workflow for generation of allogenic T cells with site-specific transgene knock-in by AsCas12a Ultra. The activated T cells were electroporated with Ultra RNPs and transduced with AAV6 carrying a donor template post-electroporation, then analyzed 3–4 days later for transgene expression by flow cytometry. D The knock-in efficiency of a fluorescent reporter at either TRAC or B2M locus using 1 µM RNP and 1 × 10 5 vg/cell AAV6. The optimal gRNA targeting each locus (n = 1) was used for this experiment. Alternative RNPs (Alt RNP, i.e., TRAC RNP + B2M AAV or B2M RNP + TRAC AAV) were used as negative control to rule out any non-specific integration of AAV donor. Data are presented as mean values ± SD. Paired, two-tailed t-test was performed to evaluate statistical significance (** P -value = 0.0012; *** P -value = 0.0008). E Simultaneous double knock-in of fluorescent reporters at TRAC and B2M across a range of RNP concentrations. The optimal gRNA targeting each locus ( n = 1) was used for this experiment. Raw source data are provided in the Source Data File.

    Techniques Used: Knock-Out, Triple Knockout, Immunopeptidomics, Multiplex Assay, Knock-In, Transduction, Electroporation, Expressing, Flow Cytometry, Negative Control, Two Tailed Test

    A Potential editing strategy for the generation of edited NK cells to overcome tumor microenvironment. Knocking out TGFBR2 (Transforming growth factor-beta receptor type 2) prevents inactivation by tumor-derived TGF-β1. B Editing efficiency of TGFBR2A across a range of RNP concentrations in NK cells derived from three different donors. Data are presented as mean values ± SD. C Tumor spheroid killing mediated by TGFBR2 knock-out NK cells. Engineered NK cells were co-cultured with SK-OV-3 ovarian tumor spheroids at effector-to-target cells ratio of 10:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Data are representative of five independent experiments using six unique NK cell donors. D Graphical depiction of EGFR CAR NK cell recognition of an EGFR + tumor cell. E Knock-in efficiency of a fluorescent reporter at either TRAC (Target site 1 and Target site 2) or B2M (Target site 3) loci using 4 μM RNP and 2.5 × 10 4 vg/cell AAV6 titer carrying the transgenes. F Tumor spheroid killing mediated by GFP + or αEGFR CAR + NK cells. Engineered NK cells were co-cultured with EGFR + PC-3 spheroids at effector-to-target cell ratios of 2:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Raw source data are provided in the Source Data File.
    Figure Legend Snippet: A Potential editing strategy for the generation of edited NK cells to overcome tumor microenvironment. Knocking out TGFBR2 (Transforming growth factor-beta receptor type 2) prevents inactivation by tumor-derived TGF-β1. B Editing efficiency of TGFBR2A across a range of RNP concentrations in NK cells derived from three different donors. Data are presented as mean values ± SD. C Tumor spheroid killing mediated by TGFBR2 knock-out NK cells. Engineered NK cells were co-cultured with SK-OV-3 ovarian tumor spheroids at effector-to-target cells ratio of 10:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Data are representative of five independent experiments using six unique NK cell donors. D Graphical depiction of EGFR CAR NK cell recognition of an EGFR + tumor cell. E Knock-in efficiency of a fluorescent reporter at either TRAC (Target site 1 and Target site 2) or B2M (Target site 3) loci using 4 μM RNP and 2.5 × 10 4 vg/cell AAV6 titer carrying the transgenes. F Tumor spheroid killing mediated by GFP + or αEGFR CAR + NK cells. Engineered NK cells were co-cultured with EGFR + PC-3 spheroids at effector-to-target cell ratios of 2:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Raw source data are provided in the Source Data File.

    Techniques Used: Derivative Assay, Knock-Out, Cell Culture, Cell Analysis, Knock-In



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    aav6 sirion biotech  (SIRION Biotech)
    90
    SIRION Biotech aav6 sirion biotech
    A Potential editing strategy for the generation of allogenic T cells. Multiplexed knock-out of TRAC , B2M , and CIITA to reduce the risk of host-versus-graft rejection and graft-versus-host disease responses, respectively. B Simultaneous triple knockout of TRAC , B2M , and CIITA (Class II Major Histocompatibility Complex Transactivator) by AsCas12a Ultra (1 µM RNP). The optimal guide RNA ( n = 1) targeting each locus was used for multiplex editing. Data are presented as mean values ± SD. C Workflow for generation of allogenic T cells with site-specific transgene knock-in by AsCas12a Ultra. The activated T cells were electroporated with Ultra RNPs and transduced with <t>AAV6</t> carrying a donor template post-electroporation, then analyzed 3–4 days later for transgene expression by flow cytometry. D The knock-in efficiency of a fluorescent reporter at either TRAC or B2M locus using 1 µM RNP and 1 × 10 5 vg/cell AAV6. The optimal gRNA targeting each locus (n = 1) was used for this experiment. Alternative RNPs (Alt RNP, i.e., TRAC RNP + B2M AAV or B2M RNP + TRAC AAV) were used as negative control to rule out any non-specific integration of AAV donor. Data are presented as mean values ± SD. Paired, two-tailed t-test was performed to evaluate statistical significance (** P -value = 0.0012; *** P -value = 0.0008). E Simultaneous double knock-in of fluorescent reporters at TRAC and B2M across a range of RNP concentrations. The optimal gRNA targeting each locus ( n = 1) was used for this experiment. Raw source data are provided in the Source Data File.
    Aav6 Sirion Biotech, supplied by SIRION Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav6+sirion+biotech/pmc08222333-361-11-12?v=SIRION+Biotech
    Average 90 stars, based on 1 article reviews
    aav6 sirion biotech - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    A Potential editing strategy for the generation of allogenic T cells. Multiplexed knock-out of TRAC , B2M , and CIITA to reduce the risk of host-versus-graft rejection and graft-versus-host disease responses, respectively. B Simultaneous triple knockout of TRAC , B2M , and CIITA (Class II Major Histocompatibility Complex Transactivator) by AsCas12a Ultra (1 µM RNP). The optimal guide RNA ( n = 1) targeting each locus was used for multiplex editing. Data are presented as mean values ± SD. C Workflow for generation of allogenic T cells with site-specific transgene knock-in by AsCas12a Ultra. The activated T cells were electroporated with Ultra RNPs and transduced with AAV6 carrying a donor template post-electroporation, then analyzed 3–4 days later for transgene expression by flow cytometry. D The knock-in efficiency of a fluorescent reporter at either TRAC or B2M locus using 1 µM RNP and 1 × 10 5 vg/cell AAV6. The optimal gRNA targeting each locus (n = 1) was used for this experiment. Alternative RNPs (Alt RNP, i.e., TRAC RNP + B2M AAV or B2M RNP + TRAC AAV) were used as negative control to rule out any non-specific integration of AAV donor. Data are presented as mean values ± SD. Paired, two-tailed t-test was performed to evaluate statistical significance (** P -value = 0.0012; *** P -value = 0.0008). E Simultaneous double knock-in of fluorescent reporters at TRAC and B2M across a range of RNP concentrations. The optimal gRNA targeting each locus ( n = 1) was used for this experiment. Raw source data are provided in the Source Data File.

    Journal: Nature Communications

    Article Title: AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

    doi: 10.1038/s41467-021-24017-8

    Figure Lengend Snippet: A Potential editing strategy for the generation of allogenic T cells. Multiplexed knock-out of TRAC , B2M , and CIITA to reduce the risk of host-versus-graft rejection and graft-versus-host disease responses, respectively. B Simultaneous triple knockout of TRAC , B2M , and CIITA (Class II Major Histocompatibility Complex Transactivator) by AsCas12a Ultra (1 µM RNP). The optimal guide RNA ( n = 1) targeting each locus was used for multiplex editing. Data are presented as mean values ± SD. C Workflow for generation of allogenic T cells with site-specific transgene knock-in by AsCas12a Ultra. The activated T cells were electroporated with Ultra RNPs and transduced with AAV6 carrying a donor template post-electroporation, then analyzed 3–4 days later for transgene expression by flow cytometry. D The knock-in efficiency of a fluorescent reporter at either TRAC or B2M locus using 1 µM RNP and 1 × 10 5 vg/cell AAV6. The optimal gRNA targeting each locus (n = 1) was used for this experiment. Alternative RNPs (Alt RNP, i.e., TRAC RNP + B2M AAV or B2M RNP + TRAC AAV) were used as negative control to rule out any non-specific integration of AAV donor. Data are presented as mean values ± SD. Paired, two-tailed t-test was performed to evaluate statistical significance (** P -value = 0.0012; *** P -value = 0.0008). E Simultaneous double knock-in of fluorescent reporters at TRAC and B2M across a range of RNP concentrations. The optimal gRNA targeting each locus ( n = 1) was used for this experiment. Raw source data are provided in the Source Data File.

    Article Snippet: For knock-in experiments, T cells or NK cells were transduced with AAV6 (Sirion Biotech) containing the cargo of interest at the denoted MOI within 30 min after electroporation.

    Techniques: Knock-Out, Triple Knockout, Immunopeptidomics, Multiplex Assay, Knock-In, Transduction, Electroporation, Expressing, Flow Cytometry, Negative Control, Two Tailed Test

    A Potential editing strategy for the generation of edited NK cells to overcome tumor microenvironment. Knocking out TGFBR2 (Transforming growth factor-beta receptor type 2) prevents inactivation by tumor-derived TGF-β1. B Editing efficiency of TGFBR2A across a range of RNP concentrations in NK cells derived from three different donors. Data are presented as mean values ± SD. C Tumor spheroid killing mediated by TGFBR2 knock-out NK cells. Engineered NK cells were co-cultured with SK-OV-3 ovarian tumor spheroids at effector-to-target cells ratio of 10:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Data are representative of five independent experiments using six unique NK cell donors. D Graphical depiction of EGFR CAR NK cell recognition of an EGFR + tumor cell. E Knock-in efficiency of a fluorescent reporter at either TRAC (Target site 1 and Target site 2) or B2M (Target site 3) loci using 4 μM RNP and 2.5 × 10 4 vg/cell AAV6 titer carrying the transgenes. F Tumor spheroid killing mediated by GFP + or αEGFR CAR + NK cells. Engineered NK cells were co-cultured with EGFR + PC-3 spheroids at effector-to-target cell ratios of 2:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Raw source data are provided in the Source Data File.

    Journal: Nature Communications

    Article Title: AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

    doi: 10.1038/s41467-021-24017-8

    Figure Lengend Snippet: A Potential editing strategy for the generation of edited NK cells to overcome tumor microenvironment. Knocking out TGFBR2 (Transforming growth factor-beta receptor type 2) prevents inactivation by tumor-derived TGF-β1. B Editing efficiency of TGFBR2A across a range of RNP concentrations in NK cells derived from three different donors. Data are presented as mean values ± SD. C Tumor spheroid killing mediated by TGFBR2 knock-out NK cells. Engineered NK cells were co-cultured with SK-OV-3 ovarian tumor spheroids at effector-to-target cells ratio of 10:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Data are representative of five independent experiments using six unique NK cell donors. D Graphical depiction of EGFR CAR NK cell recognition of an EGFR + tumor cell. E Knock-in efficiency of a fluorescent reporter at either TRAC (Target site 1 and Target site 2) or B2M (Target site 3) loci using 4 μM RNP and 2.5 × 10 4 vg/cell AAV6 titer carrying the transgenes. F Tumor spheroid killing mediated by GFP + or αEGFR CAR + NK cells. Engineered NK cells were co-cultured with EGFR + PC-3 spheroids at effector-to-target cell ratios of 2:1 (E:T). The spheroid size was monitored over time by Incucyte real-time cell analysis as a proxy for NK cytotoxicity. Two-way ANOVA was performed to evaluate statistical significance (**** P -value ≤ 0.0001). Raw source data are provided in the Source Data File.

    Article Snippet: For knock-in experiments, T cells or NK cells were transduced with AAV6 (Sirion Biotech) containing the cargo of interest at the denoted MOI within 30 min after electroporation.

    Techniques: Derivative Assay, Knock-Out, Cell Culture, Cell Analysis, Knock-In